Mycoplasma
| Mycoplasma | |
|---|---|
| |
| Mycoplasma haemofelis | |
Scientific classification 
| |
| Domain: | Bacteria |
| Kingdom: | Bacillati |
| Phylum: | Mycoplasmatota |
| Class: | Mollicutes |
| Order: | Mycoplasmatales |
| Family: | Mycoplasmataceae |
| Genus: | Mycoplasma J.Nowak 1929 |
| Type species | |
| Mycoplasma mycoides (Borrel et al. 1910) Freundt 1955 (Approved Lists 1980)
| |
| Species | |
|
See text | |
| Synonyms | |
| |
| Mycoplasmosis | |
|---|---|
| Specialty | Infectious disease |
Mycoplasma is a genus of bacteria that, like the other members of the class Mollicutes, lack a cell wall, and its peptidoglycan, around their cell membrane.[1] The absence of peptidoglycan makes them naturally resistant to antibiotics such as the beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic.[2]
In casual speech, the name "mycoplasma" (plural mycoplasmas or mycoplasms) generally refers to all members of the class Mollicutes. In formal scientific classification, the designation Mycoplasma refers exclusively to the genus, a member of the Mycoplasmataceae, the only family in the order Mycoplasmatales (see "scientific classification"). In 2018, Mycoplasma was split with many clinically significant species moved to other genera in Mollicutes; see the page Mollicutes for an overview.
Etymology
[edit]The term "mycoplasma", from the Greek μύκης, mykes (fungus) and πλάσμα, plasma (formed), was first used by Albert Bernhard Frank in 1889 to describe an altered state of plant cell cytoplasm resulting from infiltration by fungus-like microorganisms.[3][4] Julian Nowak later proposed the name mycoplasma for certain filamentous microorganisms imagined to have both cellular and acellular stages in their lifecycles, which could explain how they were visible with a microscope, but passed through filters impermeable to other bacteria.[5]
Later, the name for these mycoplasmas was pleuropneumonia-like organisms (PPLO), broadly referring to organisms similar in colonial morphology and filterability to the causative agent (a Mycoplasma species) of contagious bovine pleuropneumonia.[6] At present, all these organisms are classified as Mollicutes, and the term Mycoplasma solely refers to the genus.[citation needed]
Characteristics
[edit]Over 100 species were formerly included in the genus Mycoplasma, a member of the class Mollicutes. They are parasites or commensals of humans, animals, and plants. The genus Mycoplasma uses vertebrate and arthropod hosts.[7] Dietary nitrogen availability has been shown to alter codon bias and genome evolution in Mycoplasma and the plant parasites Phytoplasma.[8]
Mycoplasma species are among the smallest free-living organisms (about 0.2–0.3 μm in diameter).[9][10] They have been found in the pleural cavities of cattle suffering from pleuropneumonia. These organisms are often called MLO (mycoplasma-like organisms) or, formerly, PPLO (pleuropneumonia-like organisms).[6]
Important characteristics
[edit]- Cell wall is absent and plasma membrane forms the outer boundary of the cell.
- Due to the absence of cell walls these organisms can change their shape and leads to pleomorphism.
- Lack of nucleus and other membrane-bound organelles.
- Genetic material is a single DNA duplex and is naked.
- Ribosomes are 70S type.
- Possess a replicating disc at one end which assists replication process and also the separation of the genetic materials.
- Heterotrophic nutrition. Some live as saprophytes but the majority are parasites of plants and animals. The parasitic nature is due to the inability of mycoplasmal bacteria to synthesise the required growth factor.
Cell and colony morphology
[edit]Due to the lack of a rigid cell wall, Mycoplasma species (like all Mollicutes) can contort into a broad range of shapes, from round to oblong. They are pleomorphic and therefore cannot be identified as rods, cocci or spirochetes.[11]
Colonies show the typical "fried egg" appearance (about 0.5 mm in diameter).[10]
Reproduction
[edit]In 1954, using phase-contrast microscopy, continual observations of live cells have shown that Mycoplasma species ("mycoplasmas", formerly called pleuropneumonia-like organisms, PPLO, now classified as Mollicutes) and L-form bacteria (previously also called L-phase bacteria) do not proliferate by binary fission, but by a uni- or multi-polar budding mechanism. Microphotograph series of growing microcultures of different strains of PPLOs, L-form bacteria and, as a control, a Micrococcus species (dividing by binary fission) have been presented.[10] Additionally, electron microscopic studies have been performed.[12]
Taxonomy
[edit]History of taxonomy
[edit]Previously,[when?] Mycoplasma species (often commonly called "mycoplasmas", now classified as Mollicutes) were sometimes considered stable L-form bacteria or even viruses, but phylogenetic analysis has identified them as bacteria that have lost their cell walls in the course of evolution.[13]
The medical and agricultural importance of members of the genus Mycoplasma and related genera have led to the extensive cataloging of many of these organisms by culture, serology, and small sub-unit rRNA gene and whole-genome sequencing. A recent focus in the sub-discipline of molecular phylogenetics has both clarified and confused certain aspects of the organization of the class Mollicutes.[14]
Taxonomists once[when?] classified Mycoplasma and relatives in the phylum Firmicutes, consisting of low G+C Gram-positive bacteria such as Clostridium, Lactobacillus, and Streptococcus; but modern polyphasic analyses situate them in the phylum Tenericutes.[15]
By the 1990s, it had become readily apparent that this approach was problematic: the type species, M. mycoides, along with other significant mycoplasma species like M. capricolum, is evolutionarily more closely related to the genus Spiroplasma in the order Entomoplasmatales than to the other members of the genus Mycoplasma. As a result, if the group was to be rearranged to match phylogeny, a number of medically important species (e.g. M. pneumoniae, M. genitalium) would have to be put in a different genus, causing widespread confusion in medical and agricultural communities. The genus was discussed multiple times by the International Committee on Systematic Bacteriology's (ICSB) subcommittee on Mollicutes between 1992 and 2011, to no effect.[16]
Regardless of taxonomy, by 2007 it was solidly known that Molicutes could be divided into four nontaxonomic lineages.[17][18]
- An "Acholeplasma" group consisting of Acholeplasmatales. This group is non-problematic, as it contains no species classified in what was then "Mycoplasma".
- A "Spiroplasma" or mycoides group containing M. mycoides and the aforementioned closely-related species in "Spiroplasma" and Entomoplasmatales.
- A pneumoniae group containing M. pneumoniae and closely-related species (M. muris, M. fastidiosum, U. urealyticum), the currently unculturable haemotrophic mollicutes, informally referred to as haemoplasmas (recently transferred from the genera Haemobartonella and Eperythrozoon), and Ureaplasma. This medically important group contains M. alvi (bovine), M. amphoriforme (human), M. gallisepticum (avian), M. genitalium (human), M. imitans (avian), M. pirum (uncertain/human), M. testudinis (tortoises), and M. pneumoniae (human). Most, if not all, of these species share some otherwise unique characteristics including an attachment organelle, homologs of the M. pneumoniae cytadherence-accessory proteins, and specialized modifications of the cell division apparatus.
- A hominis group containing M. hominis, M. bovis, and M. pulmonis among others.
As of 2018
[edit]In 2018, Gupta et al. re-circumscribed the genus Mycoplasma around M. mycoides. A total of 78 species were removed from Mycoplasma, creating five new genera and a number of higher taxonomic levels. Under this new scheme, a new family Mycoplasmoidaceae was created to correspond to the "pneumoniae" group, with M. pneumoniae and related species transferred to a new genus Mycoplasmoides. Another new family Metamycoplasmataceae was created to correspond to the "hominis" group. Both families belong to a new order Mycoplasmoitales, distinct from the Mycoplasmatales of Mycoplasma.[18] The taxonomy was accepted by the ICSB with validation list 184 in 2018 and became the correct name. Both List of Prokaryotic names with Standing in Nomenclature (LPSN)[16] and National Center for Biotechnology Information (NCBI) now use the new nomenclature.[19]
Gupta's proposed taxonomy, as expected, moved the medically important "pneumoniae" group out of Mycoplasma into its own genus. As a result, a number of mycoplasmologists petitioned to the ICSB to reject the name in 2019. They argue that although Gupta's phylogenetic methods were likely solid, the proposed name changes are too sweeping to be practically adopted, citing some principles of the Code such as "name stability".[20] Gupta and Oren wrote a rebuttal in 2020, further detailing the pre-existing taxonomic problems.[21][22] In 2022, the ICSP's Judicial Opinion 122 ruled in favor of the name changes proposed by Gupta, meaning they remain valid under the Prokaryotic Code[23] (and for the purpose of the LPSN, they remain the "correct names").[22] However, the older names also remain valid; their use remains acceptable under the Code.[23]
Gupta et al. 2019 performed some uncontroversial sorting of the order Mycoplasmatales.[24]
| 16S rRNA based LTP_10_2024[25][26][27] | 120 marker proteins based GTDB 09-RS220[28][29][30] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Unassigned species:
- "Ca. M. aoti" Barker et al. 2011
- "M. bradburyae" Ramírez et al. 2023
- "Ca. M. corallicola" Neulinger et al. 2009
- "Ca. M. coregoni" corrig. Rasmussen et al. 2021
- "Ca. M. didelphidis" corrig. Pontarolo et al. 2021
- "Ca. M. erythrocervae" Watanabe et al. 2010
- "Ca. M. haematobrackenitadaridae" Becker et al. 2025
- "Ca. M. haematocervi" corrig. Watanabe et al. 2010
- "Ca. M. haematodidelphidis" corrig. Messick et al. 2002
- "Ca. M. haematohominis" corrig. Millán et al. 2015
- "Ca. M. haematohydrochoeri" corrig. Vieira et al. 2021
- "Ca. M. haematomacacae" corrig. Maggi et al. 2013
- "Ca. M. haematominiopteri" corrig. Millán et al. 2015
- "Ca. M. haematomolossi" Becker et al. 2025
- "M. haematomyotis" Volokhov et al. 2023
- "Ca. M. haematonasuae" corrig. Collere et al. 2021
- "Ca. M. haematoparvum" Sykes et al. 2005
- "M. haematophyllostomi" Volokhov et al. 2023
- "Ca. M. haematoselmanitadaridae" Becker et al. 2025
- "Ca. M. haematosphigguri" corrig. Valente et al. 2021
- "Ca. M. haematotapirus" Mongruel et al. 2022
- "Ca. M. haematoterrestris" Mongruel et al. 2022
- "Ca. M. haematotraderitadaridae" Becker et al. 2025
- "Ca. M. haematovis" corrig. Hornok et al. 2009
- "Ca. M. haemobovis" Meli et al. 2010
- "Ca. M. haemomeles" Harasawa, Orusa & Giangaspero 2014
- "Ca. M. haemomuris" (Mayer 1921) Neimark et al. 2002
- "Ca. M. haemoparvum" Kenny et al. 2004
- M. hafezii Ziegler et al. 2019
- "Ca. M. hemominiopterus" Millán et al. 2015
- "M. incognitus" Lo et al. 1989
- "M. insons" May et al. 2007
- "Ca. M. kahanei" Neimark et al. 2002
- "M. monodon" Ghadersohi & Owens 1998
- M. phocimorsus Skafte-Holm et al. 2023
- "M. pneumophila" Lyerova et al. 2008
- "Ca. M. ravipulmonis" Neimark, Mitchelmore & Leach 1998
- "Ca. M. salmoniarum" corrig. Rasmussen et al. 2021
- "M. sphenisci" Frasca et al. 2005
- "M. timone" Greub & Raoult 2001
- "Ca. M. tructae" Sanchez et al. 2020
- "Ca. M. turicense" corrig. Willi et al. 2006
- "M. volis" Dillehay et al. 1995
- "M. vulturii" Oaks et al. 2004
Synthetic mycoplasma genome
[edit]A chemically synthesized genome of a mycoplasmal cell based entirely on synthetic DNA which can self-replicate has been referred to as Mycoplasma laboratorium.[33] It is therefore a model for a minimal genome, that is, a genome that is reduced to mostly essential genes.
See also
[edit]- International Organization for Mycoplasmology (IOM)
- Sexually transmitted disease
- Vaginal flora
- Vaginal infection
- Vaginal disease
- Vaginal health
- Phytoplasma
- Smallest organisms
- List of bacterial orders
- List of bacteria genera
- Mycoplasma alligatori
References
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From p. 335: "Die durch die Infection entstandene veränderte Art des Plasmas in den Rindenzellen will ich, da sie offenbar durch die Vermischmung mit einem pilzartigen Wesen entstanden ist, als Mycoplasma bezeichnen." [I want to designate as "mycoplasma" the altered type of plasma in the cortex cells which arose by infection, since it [i.e., the altered type of plasma] obviously arose by mixing with a fungal organism.]
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- ^ Kandler G, Kandler O, Huber O (1954). "Untersuchungen über die Morphologie und die Vermehrung der pleuropneumonie-ähnlichen Organismen und der L-Phase der Bakterien. II. Elektronenmikroskopische Untersuchungen" [Studies on morphology and multiplication of pleuropneumonia-like organisms and on bacterial L-phase, II. Electron microscopy (now mycoplasmas and L-form bacteria)] (PDF). Archiv für Mikrobiologie (in German). 21 (2). (Article in English available): 202–216. Bibcode:1954ArMic..21..202K. doi:10.1007/BF01816379. PMID 14350642. S2CID 45546531.
- ^ Woese CR, Maniloff J, Zablen L (1980). "Phylogenetic analysis of the mycoplasmas" (PDF). Proceedings of the National Academy of Sciences of the United States of America. 77 (1): 494–498. Bibcode:1980PNAS...77..494W. doi:10.1073/pnas.77.1.494. PMID 692864.
- ^ Johansson K-E, Pettersson B (2002). "Taxonomy of Mollicutes". Molecular Biology and Pathogenicity of Mycoplasmas (Razin S, Herrmann R, eds.). New York: Kluwer Academic/Plenum. pp. 1–30. ISBN 0-306-47287-2.
- ^ Brown DR (2011). "Phylum XVI. Tenericutes Murray 1984a, 356VP". Bergey's Manual of Systematic Bacteriology, Second Edition, Vol. 4, (Krieg NR, Staley JT, Brown DR, et al., eds.). New York: Springer. pp. 567–568. ISBN 978-0-387-95042-6.
- ^ a b A.C. Parte, et al. "Mycoplasma". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved 9 September 2022.
- ^ Oshima K, Nishida H (September 2007). "Phylogenetic relationships among mycoplasmas based on the whole genomic information". J. Mol. Evol. 65 (3): 249–58. Bibcode:2007JMolE..65..249O. doi:10.1007/s00239-007-9010-3. PMID 17687503.
- ^ a b Gupta R, Sawnani S, Adeolu M, Alnajar S, Oren A (2018). "Phylogenetic framework for the phylum Tenericutes based on genome sequence data: proposal for the creation of a new order Mycoplasmoidales ord. nov., containing two new families Mycoplasmoidaceae fam. nov. and Metamycoplasmataceae fam. nov. harbouring Eperythrozoon, Ureaplasma and five novel genera". Antonie van Leeuwenhoek. 111 (9): 1583–1630. doi:10.1007/s10482-018-1047-3. PMID 29556819. S2CID 254226604.
- ^ Sayers, et al. "Mycoplasma". National Center for Biotechnology Information (NCBI) taxonomy database. Retrieved 9 September 2022.
- ^ Balish M, Bertaccini A, Blanchard A, Brown D, Browning G, Chalker V, Frey J, Gasparich G, Hoelzle L, Knight T, Knox C, Chih-Horng K, Manso-Silván L, May M, Pollack J, Ramírez A, Spergser J, Taylor-Robinson D, Volokhov D, Zhao Y (2019). "Recommended rejection of the names Malacoplasma gen. nov., Mesomycoplasma gen. nov., Metamycoplasma gen. nov., Metamycoplasmataceae fam. nov., Mycoplasmoidaceae fam. nov., Mycoplasmoidales ord. nov., Mycoplasmoides gen. nov., Mycoplasmopsis gen. nov. [Gupta, Sawnani, Adeolu, Alnajar and Oren 2018] and all proposed species comb. nov. placed therein". International Journal of Systematic and Evolutionary Microbiology. 69 (11): 3650–3653. doi:10.1099/ijsem.0.003632. hdl:11585/720151. PMID 31385780.
- ^ Gupta RS, Oren A (1 February 2020). "Necessity and rationale for the proposed name changes in the classification of Mollicutes species. Reply to: 'Recommended rejection of the names Malacoplasma gen. nov., Mesomycoplasma gen. nov., Metamycoplasma gen. nov., Metamycoplasmataceae fam. nov., Mycoplasmoidaceae fam. nov., Mycoplasmoidales ord. nov., Mycoplasmoides gen. nov., Mycoplasmopsis gen. nov. [Gupta, Sawnani, Adeolu, Alnajar and Oren 2018] and all proposed species comb. nov. placed therein', by M. Balish et al. (Int J Syst Evol Microbiol, 2019;69:3650–3653)". International Journal of Systematic and Evolutionary Microbiology. 70 (2): 1431–1438. doi:10.1099/ijsem.0.003869. PMID 31971499.
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- ^ Gupta RS, Son J, Oren A (April 2019). "A phylogenomic and molecular markers based taxonomic framework for members of the order Entomoplasmatales: proposal for an emended order Mycoplasmatales containing the family Spiroplasmataceae and emended family Mycoplasmataceae comprising six genera". Antonie van Leeuwenhoek. 112 (4): 561–588. doi:10.1007/s10482-018-1188-4. PMID 30392177.
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