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| Players
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Coaches/Other
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Pitchers
- 54 Will Armbruester
- 18 Hendry Chivilli
- 68 Miguel Cordero
- 62 Juan Cota
- 25 Joel Garcia
- 19 Ruddy Gomez
- 31 Andrew Huffman
- 33 Xavier Kolhosser
- 37 Eider Machuca
- 39 Mitch Mueller
- 60 Sebastian Pulido
- 64 Anderson Ramos
- 26 Liam Rocha
- 45 Melvin Rodriguez
- 43 Santiago Rojas
- 56 Leonardo Rondon
- 24 Yoel Roque
- 20 Aiberson Ventura
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Catchers
- 59 Victor Leal
- 22 Irvin Nunez
- 71 Ricardo Pena
- 27 Javier Roman
- 9 Carlos Silva
Infielders
- 13 Bryan Acuna
- 23 Daiber De Los Santos
- 8 Ramiro Dominguez
- 61 Yilber Herrera
- 16 Isaac Pena
Outfielders
- 1 Jayson Bass
- 44 Eduardo Beltre
- 17 Ariel Castro
- 19 Gregory Duran
- 50 Luis Fragoza
- 2 Merphy Hernandez
- 55 Yandro Hernandez
- 5 Ricardo Paez
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Manager
Coaches
- 93 Emilio Guerrero (hitting)
- -- Erick Julio (pitching)
- -- Carter Kessinger (rehab pitching)
- -- Collin McBride (hitting)
- 99 Humberto Miranda (hitting)
- 96 Chris Powell (pitching)
- -- Dan Urbina (pitching)
60-day injured list
- 57 Carlos Gutierrez (full season)
 7-day injured list
* On Minnesota Twins 40-man roster
~ Development list
# Rehab assignment
∞ Reserve list
‡ Restricted list
§ Suspended list
† Temporarily inactive list
Roster updated May 2, 2025
Transactions
→ More rosters: MiLB • Florida Complex League
→ Minnesota Twins minor league players
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